Open AccessImpact of B ifidobacterium bifidum MIMB b75 on mouse intestinal microorganisms
Author(s) -
Singh Natasha,
Arioli Stefania,
Wang Angela,
Villa Christopher R.,
Jahani Raha,
Song Ye Seul,
Mora Diego,
Guglielmetti Simone,
Comelli Elena M.
Publication year - 2013
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12124
Subject(s) - bifidobacterium bifidum , biology , microbiology and biotechnology , bifidobacterium , cecum , probiotic , feces , clostridium , bacteria , lactobacillus , genetics , ecology
B ifidobacterium bifidum MIMB b75 is a recently identified probiotic. However, its distribution along the intestine and impact on resident microbiota is unknown. Herein, we established a quantitative real‐time polymerase chain reaction assay targeting the B . bifidum ‐specific BopA region for the quantification of B . bifidum in feces and used this assay to investigate transit of B . bifidum MIMB b75 through the murine intestine. We also analyzed the consequential impact on resident microbial cohorts. C 57 BL /6 J mice were daily gavaged with 0.2 mL of either sterile PBS or PBS containing 10 8 colony‐forming units of B . bifidum MIMB b75 for 2 weeks, after which intestinal contents and fecal samples were analyzed for microbial compositional changes. B ifidobacterium bifidum MIMB b75 was able to transiently colonize the murine intestine, with the predominant niche being the ceco‐proximal colonic region. Region‐specific effects on host microbiota were observed including decreased levels of C lostridium coccoides in the cecum, increased levels of bifidobacteria in the proximal and distal colon, total bacteria and C lostridium leptum in the proximal colon, and of C . coccoides in the feces. These findings suggest that probiotic properties of B . bifidum MIMB b75 may partially depend on its ability to at least transiently colonize the intestine and impact on the resident microbial communities at various intestinal loci.