Open AccessBacterioplankton communities turn unstable and become small under increased temperature and nutrient‐enriched conditions
Author(s) -
Ren Lijuan,
He Dan,
Zeng Jin,
Wu Qinglong L.
Publication year - 2013
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12089
Subject(s) - microcosm , bacterioplankton , biology , nutrient , temperature gradient gel electrophoresis , ecology , actinobacteria , microbial population biology , community structure , bacteria , 16s ribosomal rna , phytoplankton , genetics
The stability of microbial community composition under different environmental conditions is an important part of microbial ecology, but has not been investigated in such depth before. In this study, we investigated the composition of the bacterioplankton community composition ( BCC ) and its stability under different temperatures (15, 25 and 35 °C, respectively) and nutrient conditions (control vs. nitrogen‐ and phosphorus‐enriched) in aquatic microcosms. The BCC was analysed using denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene, followed by cloning and sequence analysis. BCC in the microcosms significantly changed under different temperature and nutrient conditions. Based on the results from clone libraries, ecological diversification were observed within two ubiquitous and dominant genera, M ethylophilus and P olynucleobacter , under different temperature and nutrient conditions. Putative ultramicrobacteria, which included A ctinobacteria , P olynucleobacter sp., LD 12 and LD 28 clusters and bacteria affiliated with subcluster I of M ethylophilus , were found to dominate in bacterioplankton communities at higher temperatures (25 and 35 °C), regardless of nutrient conditions. We also observed that the rate of BCC change increased at higher temperatures and this increase was more pronounced in nutrient‐enriched microcosms. These results indicated that bacterioplankton communities become unstable and decrease in size with increased temperature and in nutrient‐enriched conditions.