Open AccessInfluence of bacterial communities based on 454‐pyrosequencing on the survival of E scherichia coli O157:H7 in soils
Author(s) -
Ma Jincai,
Ibekwe Abasiofiok M.,
Yang ChingHong,
Crowley David E.
Publication year - 2013
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12083
Subject(s) - acidobacteria , biology , actinobacteria , pyrosequencing , bacteroidetes , proteobacteria , escherichia coli , soil water , microbiology and biotechnology , bacteria , pathogen , bacterial phyla , botany , 16s ribosomal rna , ecology , biochemistry , genetics , gene
Shiga toxin‐producing Escherichia coli O 157: H 7 has been implicated in many foodborne illnesses. In this study, survival of E . coli O 157: H 7 in 32 soils from C alifornia ( CA ) and A rizona ( AZ ) was investigated. Our goal was to correlate the survival time of E . coli O 157: H 7 in soils with 16S r RNA pyrosequencing based bacterial community composition. Kohonen self‐organizing map of survival and associated soil chemical, physical and biological variables using artificial neural network analysis showed that survival of E . coli O 157: H 7 in soils was negatively correlated with salinity ( EC ), but positively correlated with total nitrogen ( TN ) and water soluble organic carbon ( WSOC ). Bacterial diversity as determined by the Shannon diversity index had no significant ( P = 0.635) effect on ttd , but individual bacterial phyla had different effects. The survival of E . coli O 157: H 7 was positively correlated with the abundances of A ctinobacteria ( P < 0.001) and A cidobacteria ( P < 0.05), and negatively correlated with those of P roteobacteria and B acteroidetes ( P < 0.05). Our data showed that specific groups of bacteria correlate with the persistence of E . coli O 157: H 7 in soils thus opening new ways to study the influence of certain bacterial phyla on persistence of this pathogen and other related pathogens in complex environments.