Open AccessEthylene production in relation to nitrogen metabolism in Saccharomyces cerevisiae
Author(s) -
Johansson Nina,
Persson Karl O.,
Quehl Paul,
Norbeck Joakim,
Larsson Christer
Publication year - 2014
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12208
Subject(s) - biology , saccharomyces cerevisiae , nitrogen cycle , metabolism , relation (database) , nitrogen , production (economics) , ethylene , saccharomyces , biochemistry , yeast , chemistry , economics , computer science , data mining , catalysis , microeconomics , organic chemistry
We have previously shown that ethylene production in Saccharomyces cerevisiae expressing the ethylene‐forming enzyme ( EFE ) from Pseudomonas syringae is strongly influenced by variations in the mode of cultivation as well as the choice of nitrogen source. Here, we have studied the influence of nitrogen metabolism on the production of ethylene further. Using ammonium, glutamate, glutamate/arginine, and arginine as nitrogen sources, it was found that glutamate (with or without arginine) correlates with a high ethylene production, most likely linked to an observed increase in 2‐oxoglutarate levels. Arginine as a sole nitrogen source caused a reduced ethylene production. A reduction of arginine levels, accomplished using an arginine auxotrophic ARG 4 ‐deletion strain in the presence of limiting amounts of arginine or through CAR 1 overexpression, did however not correlate with an increased ethylene production. As expected, arginine was necessary for ethylene production as ethylene production in the ARG 4 ‐deletion strain ceased at the time when arginine was depleted. In conclusion, our data suggest that high levels of 2‐oxoglutarate and a limited amount of arginine are required for successful ethylene production in yeast.