Open AccessThe yeast oligopeptide transporter Opt2 is localized to peroxisomes and affects glutathione redox homeostasis
Author(s) -
ElbazAlon Yael,
Morgan Bruce,
Clancy Anne,
Amoako Theresa N.E.,
Zalckvar Einat,
Dick Tobias P.,
Schwappach Blanche,
Schuldiner Maya
Publication year - 2014
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12196
Subject(s) - peroxisome , glutathione , biology , cytosol , microbiology and biotechnology , homeostasis , mitochondrion , biochemistry , organelle , gene , enzyme
Glutathione, the most abundant small‐molecule thiol in eukaryotic cells, is synthesized de novo solely in the cytosol and must subsequently be transported to other cellular compartments. The mechanisms of glutathione transport into and out of organelles remain largely unclear. We show that budding yeast Opt2, a close homolog of the plasma membrane glutathione transporter Opt1, localizes to peroxisomes. We demonstrate that deletion of OPT2 leads to major defects in maintaining peroxisomal, mitochondrial, and cytosolic glutathione redox homeostasis. Furthermore, ∆opt2 strains display synthetic lethality with deletions of genes central to iron homeostasis that require mitochondrial glutathione redox homeostasis. Our results shed new light on the importance of peroxisomes in cellular glutathione homeostasis.