Open AccessMechanosensitive channels Msy1 and Msy2 are required for maintaining organelle integrity upon hypoosmotic shock in Schizosaccharomyces pombe
Author(s) -
Nakayama Yoshitaka,
Hirata Aiko,
Iida Hidetoshi
Publication year - 2014
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12181
Subject(s) - mechanosensitive channels , biology , microbiology and biotechnology , schizosaccharomyces pombe , vacuole , endoplasmic reticulum , organelle , cytoplasm , schizosaccharomyces , mutant , yeast , biophysics , saccharomyces cerevisiae , biochemistry , ion channel , receptor , gene
The mechanosensitive channels, Mys1 and Msy2, in fission yeast are localized in the endoplasmic reticulum membrane and control cytoplasmic Ca 2+ levels in the hypoosmotic response. We here investigated changes in organellar structures with hypoosmotic shock using transmission electron microscopy. While msy1 − and msy2 − single mutant cells developed a number of swollen vacuoles following hypoosmotic shock, similar to wild‐type cells, msy1 − msy2 − double mutant cells only had two abnormally large vacuoles and cracks between the inner and outer nuclear membranes. These results suggest that Msy1 and Msy2 may be involved in maintaining vacuole integrity and protecting the nuclear envelope upon hypoosmotic shock and also that these two channels are functionally complementary.