Open AccessC bc2p, U pf3p and e IF 4 G are components of the DRN (Degradation of m RNA in the Nucleus) in S accharomyces cerevisiae
Author(s) -
Das Satarupa,
Saha Upasana,
Das Biswadip
Publication year - 2014
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12180
Subject(s) - biology , saccharomyces cerevisiae , exoribonuclease , nonsense mediated decay , messenger rna , mutant , microbiology and biotechnology , eif4g , p bodies , rna , nucleus , gene , genetics , rna splicing , rnase p , translation (biology)
Abstract Messenger RNAs retained in the nucleus of Saccharomyces cerevisiae are subjected to a degradation system designated DRN ( D egradation of m R NA in the N ucleus) that is dependent on the nuclear mRNA cap‐binding protein, Cbc1p, as well as nuclear exosome component Rrp6p, a 3′ to 5′ exoribonuclease. DRN has been shown to act on RNAs preferentially retained in the nucleus, such as: (1) global mRNA s in export defective nup116‐ Δ mutant strains at the restrictive temperature; (2) a certain class of normal mRNA s called special mRNA s (e.g. IMP3 and YLR194 c mRNA s); and (3) mutant mRNA s for example, lys2‐187 and cyc1‐512 . In this study, we further identify three novel components of DRN (Cbc2p, Upf3p and Tif4631p) by employing a genetic screen and by considering proteins/factors that interact with Cbc1p. Participation of these components in DRN was confirmed by demonstrating that null alleles of these genes resulted in stabilization of the rapid decay of global mRNA s in the export defective nup116‐ Δ strain and of representative special mRNA s. Depletion of Tif4632p, an isoform of Tif4631p, also exhibited a partial impairment of DRN function and is therefore also considered to play a functional role in DRN. These findings clearly establish that CBC2 , UPF3, and TIF4631/32 gene products participate in DRN function.