Open AccessThe fatty acid transport protein Fat1p is involved in the export of fatty acids from lipid bodies in Yarrowia lipolytica
Author(s) -
Dulermo Rémi,
GamboaMeléndez Heber,
Dulermo Thierry,
Thevenieau France,
Nicaud JeanMarc
Publication year - 2014
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12177
Subject(s) - yarrowia , biochemistry , biology , fatty acid , yeast , saccharomyces cerevisiae , peroxisome , lipid droplet , free fatty acid receptor , polyunsaturated fatty acid , gene
In order to live, cells need to import different molecules, such as sugars, amino acids or lipids, using transporters. In Saccharomyces cerevisiae , the Sc FAT 1 gene encodes the long‐chain fatty acid transporter; however, the transport of fatty acids (FAs) in the oleaginous yeast Yarrowia lipolytica has not yet been studied. In contrast to what has previously been found for Δ Scfat1 strains, Δ Ylfat1 yeast was still able to grow on substrates containing short‐, medium‐ or long‐chain FAs. We observed a notable difference in cell lipid content between wild‐type (WT) and deletion mutant strains after 24 h of culture in minimal oleate medium: in the WT strain, lipids represented 24% of cell dry weight (CDW), while they accounted for 37% of CDW in the Δ Ylfat1 strain. This result indicates that YlFat1p is not involved in cell lipid uptake. Moreover, we also observed that fatty acid remobilisation was decreased in the Δ Ylfat1 strain and that fluorescence‐tagged YlFat1p proteins localised to the interfaces between lipid bodies, which suggests that YlFat1p may play a role in the export of FAs from lipid bodies.