Open AccessA tg21 regulates pexophagy via its PI (3) P ‐binding activity in P ichia pastoris
Author(s) -
Tamura Naoki,
Oku Masahide,
Sakai Yasuyoshi
Publication year - 2014
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12132
Subject(s) - pichia pastoris , biology , pi , pichia , biochemistry , microbiology and biotechnology , recombinant dna , gene
Pexophagy is a selective degradation pathway of peroxisomes. In the present study, we revealed that P p A tg21 was required for pexophagy in the methylotrophic yeast P ichia pastoris . P p A tg21 was essential for efficient lipidation of A tg8 and for de novo synthesis of pexophagic membranes. In contrast to P p A tg18, P p A tg21 was not necessary for vacuolar fission nor invagination during micropexophagy. P p A tg21 specifically bound to PI (3) P , but not to PI (3,5) P 2 in vitro , and the localization analyses matched with this phosphoinositide‐binding specificity. The mutant which lost the lipid‐binding activity showed defect in pexophagy, suggesting that PI (3) P ‐binding activity of P p A tg21 was required for pexophagy.