Open AccessENV 7 and YCK 3 , which encode vacuolar membrane protein kinases, genetically interact to impact cell fitness and vacuole morphology
Author(s) -
Manandhar Surya P.,
Gharakhanian Editte
Publication year - 2014
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12128
Subject(s) - vacuole , biology , microbiology and biotechnology , saccharomyces cerevisiae , lipid bilayer fusion , kinase , protein kinase a , mutant , gene , biochemistry , membrane , cytoplasm
Saccharomyces cerevisiae vacuoles serve as a model for membrane fusion and fission. Yck3, a vacuolar membrane kinase, has been implicated in regulation of vacuole fusion. Recently, we established Env7 as another vacuolar membrane protein kinase with similar but nonredundant function to Yck3. Here, we report that native Env7 localizes to the vacuole independent of Yck3, where as its phosphorylation is YCK 3 dependent. We also show that env7 Δ yck3 Δ double mutant exhibits severely compromised fitness, altered cell size and bud vacuoles, and F‐class vacuolar morphology. Our results establish negative genetic interactions between ENV 7 and YCK 3 and suggest cooperative roles for the two conserved genes in regulation of membrane dynamics. Such genetic buffering supports a critical role for membrane flux in global cell fitness.