Open AccessAn autonomously replicating sequence for use in a wide range of budding yeasts
Author(s) -
Liachko Ivan,
Dunham Maitreya J.
Publication year - 2014
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12123
Subject(s) - biology , autonomously replicating sequence , plasmid , budding yeast , yeast , genetics , origin of replication , saccharomyces cerevisiae , sequence (biology) , whole genome sequencing , genome , kluyveromyces , dna replication , dna , gene
The initiation of DNA replication at replication origins is essential for the duplication of genomes. In yeast, the autonomously replicating sequence ( ARS ) property of replication origins is necessary for the stable maintenance of episomal plasmids. However, because the sequence determinants of ARS function differ among yeast species, current ARS modules are limited for use to a subset of yeasts. Here, we describe a short ARS sequence that functions in at least 10 diverse species of budding yeast. These include, but are not limited to members of the S accharomyces , L achancea , K luyveromyces , and P ichia ( K omagataella) genera spanning over 500 million years of evolution. In addition to its wide species range, this ARS and an optimized derivative confer improved plasmid stability relative to other currently used ARS modules.