Human ribosomal protein L 9 is a B ax suppressor that promotes cell survival in yeast

The identification of a human ribosomal protein L 9 (h RPL 9) c DNA as a sequence capable of suppressing the lethal effects of heterologously expressed murine B ax in yeast led us to investigate its antiapoptotic potential. Using growth and viability assays, we show that yeast cells heterologously expressing h RPL 9 are resistant to the growth inhibitory and lethal effects of exogenously supplied copper, indicating that it has pro‐survival properties. To explore potential mechanisms, we used yeast mutants defective in all three types of programmed cell death (apoptosis, necrosis, and autophagy). The ability to retain pro‐survival function in all the mutants suggests that h RPL 9 may regulate a common pro‐death process. In contrast, the yeast RPL9 orthologues, RPL9A and RPL9B , have opposite effects when overexpressed in yeast. In effect, instead of showing resistance to stress, RPL9A and RPL9B overexpressing cells show reduced cell growth. Further analysis indicates that the effects of overexpressed RPL9A and RPL9B are not in themselves lethal, instead, they serve to increase cell doubling time. Thus, yeast RPL9 s are more representative of RP s whose extra‐ribosomal function is similar to that of tumor suppressors. Taken together, our results demonstrate that RPL9 represents a species‐ and sequence‐specific regulator of cell growth and survival.