Open AccessNegative control glucose dependent mediated by the P reS 2 region on the translation efficiency of the reporter S h‐bleomycin gene in S accharomyces cerevisiae
Author(s) -
HadijiAbbes Nadia,
BorchaniChabchoub Istabrak,
Gargouri Ali,
MokdadGargouri Raja
Publication year - 2014
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12117
Subject(s) - biology , reporter gene , saccharomyces cerevisiae , microbiology and biotechnology , gene , translation (biology) , messenger rna , gene expression , genetics
S accharomyces cerevisiae is able to sense and respond to environmental changes such as the availability of carbon sources. In a previous work, we showed that the expression of the P re S 2‐ S gene of HBV in yeast was negatively regulated at the translational level dependent of glucose. In this study, we show that the S m RNA is detected in the polysomes indicating its active translation, while the P re S 2‐ S m RNA was mainly found in monosomes. Moreover, we used the gene reporter assay based on Z eocin resistance, to better characterize the P re S 2 region responsible for this control. Two chimeric genes composed of the N‐ and C‐terminal part of the P re S 2 fused to the S h‐bleomycin gene conferring the resistance to Z eocin were expressed in yeast. We found that the strain expressing the N‐terminal part of the P re S 2 was sensitive to Z eocin on rich medium with 2% glucose. In contrast, the strain harbouring the C‐terminal part of the P re S 2 fused to the S h‐bleomycin grew on Z eocin, indicating that the S h‐bleomycin m RNA is efficiently translated, subsequently conferring resistance to Z eocin. Our data suggest the establishment of a translational control via the N‐terminal part of the P re S 2 mediated by the presence of 2% glucose in the media.