Open AccessDefects in mitochondrial distribution during the prolonged lag phase of S accharomyces cerevisiae preceding growth in glycerol as the sole source of carbon
Author(s) -
McInnes Joseph,
Rehders Maren,
McFalineFigueroa José R.,
Brix Klaudia,
Pon Liza A.,
Nevoigt Elke
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12085
Subject(s) - biology , mitochondrion , saccharomyces cerevisiae , glycerol , yeast , biochemistry , respiration , microbiology and biotechnology , strain (injury) , growth medium , carbon source , biophysics , botany , genetics , anatomy , bacteria
The S accharomyces cerevisiae strain CBS 6412 has been shown to be able to grow in synthetic medium containing glycerol as the sole carbon source, conditions under which laboratory strains such as CEN . PK and S 288c cannot grow. Nonetheless, this strain exhibits a lag phase of c . 30–40 h following transition to glycerol medium. As mitochondria play a critical role in the dissimilation of the respiratory carbon source glycerol, we investigated mitochondrial function and dynamics throughout the lag phase using mitochondria‐targeted ro GFP , a redox‐sensitive GFP variant. We found that following transition to glycerol medium, mitochondria become more oxidizing, accumulate near the bud neck, and exhibit decreased inheritance into daughter cells. Directly preceding entry into exponential growth phase, mitochondria become more reducing, mitochondrial accumulations at the bud neck decrease, and inheritance of mitochondria into daughter cells is restored.