Open AccessEvolutionary engineering and transcriptomic analysis of nickel‐resistant S accharomyces cerevisiae
Author(s) -
Küçükgöze Gökhan,
Alkım Ceren,
Yılmaz Ülkü,
Kısakesen H. İbrahim,
Gündüz Sema,
Akman Süleyman,
Çakar Z. Petek
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12073
Subject(s) - nickel , saccharomyces cerevisiae , biology , cobalt , transcriptome , oxidative stress , mutant , strain (injury) , genetics , gene , biochemistry , materials science , metallurgy , gene expression , anatomy
Increased exposure to nickel compounds and alloys due to industrial development has resulted in nickel pollution and many pathological effects on human health. However, there is very limited information about nickel response, transport, and tolerance in eukaryotes. To investigate nickel resistance in the model eukaryote S accharomyces cerevisiae , evolutionary engineering by batch selection under gradually increasing nickel stress levels was performed. Nickel hyper‐resistant mutants that could resist up to 5.3 mM NiCl 2 , a lethal level for the reference strain, were selected. The mutants were also cross‐resistant against iron, cobalt, zinc, and manganese stresses and accumulated more than twofold higher nickel than the reference strain. Global transcriptomic analysis revealed that 640 upregulated genes were related to iron homeostasis, stress response, and oxidative damage, implying that nickel resistance may share common mechanisms with iron and cobalt resistance, general stress response, and oxidative damage.