Open AccessXYLH encodes a xylose/H + symporter from the highly related yeast species Debaryomyces fabryi and Debaryomyces hansenii
Author(s) -
Ferreira Danielly,
Nobre Alexandra,
Silva Marta Luisa,
FariaOliveira Fábio,
Tulha Joana,
Ferreira Célia,
Lucas Cândida
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12061
Subject(s) - debaryomyces hansenii , symporter , major facilitator superfamily , biology , biochemistry , yeast , xylose , permease , lactose permease , saccharomyces cerevisiae , gene , transporter , fermentation
The closely related yeasts Debaryomyces fabryi and Debaryomyces hansenii are excellent xylose consumers. We previously described the activity of a high‐affinity xylose/H + symport from an industrial strain of D. hansenii subsequently reclassified as D. fabryi . We now report the identification of the gene encoding this permease, AY347871.2. This was retrieved from D. fabryi gDNA using a degenerate primer PCR strategy, based on conserved regions from the amino acid sequences of three well‐characterized bacterial xylose/H + symporters. This sequence is 86% identical to another, DEHA2C11374p from D. hansenii type strain. DEHA2C11374p was conceptually ascribed to the major facilitator superfamily. The putative amino acid sequence of AY347871.2 and DEHA2C11374p presented a hydrophobicity pattern compatible with plasma membrane proteins. The last was functionally expressed in Saccharomyces cerevisiae . The sensitivity of transport activity to a protonophore confirmed its dependence on proton motive force, as expected from a symporter. We named D. fabryi AY347871.2 and D. hansenii DEHA2C11374p as XYLH from Xy lose/ H + symport. Based on the very high similarity, we suggested that Scheffersomyces stipitis Xut3 and Aspergillus nidulans AN8400.2 may also encode xylose high‐affinity permeases.