Open AccessPrimers for fourteen protein‐coding genes and the deep phylogeny of the true yeasts
Author(s) -
Koufopanou Vassiliki,
Swire Jonathan,
Lomas Susan,
Burt Austin
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12059
Subject(s) - biology , phylogenetic tree , phylogenetics , gene , genome , genetics , evolutionary biology , coding region , computational biology
The S accharomycetales or ‘true yeasts’ consist of more than 800 described species, including many of scientific, medical and commercial importance. Considerable progress has been made in determining the phylogenetic relationships of these species, largely based on r DNA sequences, but many nodes for early‐diverging lineages cannot be resolved with r DNA alone. r DNA is also not ideal for delineating recently diverged species. From published full‐genome sequence data, we have identified 14 regions of protein‐coding genes that can be PCR ‐amplified in a large proportion of a diverse collection of 25 yeast species using degenerate primers. Phylogenetic analysis of the sequences thus obtained reveals a well‐resolved phylogeny of the S accharomycetales with many branches having high bootstrap support. Analysis of published sequences from the S accharomyces paradoxus species complex shows that these protein‐coding gene fragments are also informative about genealogical relationships amongst closely related strains. Our set of protein‐coding gene fragments is therefore suitable for analysing both ancient and recent evolutionary relationships amongst yeasts.