Open AccessThe RAD 52 ortholog of Yarrowia lipolytica is essential for nuclear integrity and DNA repair
Author(s) -
CamposGóngora Eduardo,
Andaluz Encarnación,
Bellido Alberto,
RuizHerrera José,
Larriba German
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12047
Subject(s) - yarrowia , biology , rad52 , dna repair , dna , nuclear dna , genetics , rad51 , microbiology and biotechnology , yeast , gene , mitochondrial dna
Y arrowia lipolytica (Yl) is a dimorphic fungus that has become a well‐established model for a number of biological processes, including secretion of heterologous and chimerical proteins. However, little is known on the recombination machinery responsible for the integration in the genome of the exogenous DNA encoding for those proteins. We have carried out a phenotypic analysis of rad52 deletants of Y . lipolytica . YlRad52 exhibited 20–30% identity with Rad52 homologues of other eukaryotes, including S accharomyces cerevisiae and C andida albicans . Yl rad52 ‐Δ strains formed colonies on YPD ‐agar plates which were spinier and smaller than those from wild type, whereas in YPD liquid cultures they exhibited a decreased grow rate and contained cells with aberrant morphology and fragmented chromatin, supporting a role for homologous recombination ( HR ) in genome stability under nondamaging conditions. In addition, Yl rad52 mutants showed moderate to high sensitivity to UV light, oxidizing agents and compounds that cause single‐ ( SSB ) and double‐strand breaks ( DSB ), indicating an important role for Rad52 in DNA repair. These findings extend to Yl previous observations indicating that RAD 52 is a crucial gene for DNA repair in other fungi, including S. cerevisiae , C. albicans and S chizosaccharomyces pombe .