Open AccessMitochondrial signaling in S accharomyces cerevisiae pseudohyphae formation induced by butanol
Author(s) -
Starovoytova An.,
Sorokin Maxim I.,
Sokolov Svyatoslav S.,
Severin Fedor F.,
Knorre Dmitry A.
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12039
Subject(s) - biology , saccharomyces cerevisiae , mitochondrion , microbiology and biotechnology , biochemistry , yeast , oligomycin , enzyme , atpase
Yeasts growing limited for nitrogen source or treated with fusel alcohols form elongated cells – pseudohyphae. Absence of mitochondrial DNA or anaerobic conditions inhibits this process, but the precise role of mitochondria is not clear. We found that a significant percentage of pseudohyphal cells contained mitochondria with different levels of membrane potential within one cell. An uncoupler carbonyl cyanide p ‐(trifluoromethoxy) phenylhydrazone ( FCCP ), but not the ATP ‐synthase inhibitor oligomycin D , prevented pseudohyphal growth. Interestingly, repression of the MIH1 gene encoding phosphatase activator of the G 2/ M transition partially restores the ability of yeast to form pseudohyphal cells in the presence of FCCP or in the absence of mitochondrial DNA . At the same time, retrograde signaling (the one triggered by dysfunctional mitochondria) appeared to be a positive regulator of butanol‐induced pseudohyphae formation: the deletion of any of the retrograde signaling genes ( RTG1 , RTG2 , or RTG3 ) partially suppressed pseudohyphal growth. Together, our data suggest that two subpopulations of mitochondria are required for filamentous growth: one with high and another with low transmembrane potential. These mitochondria‐activated signaling pathways appear to converge at M ih1p level.