Open AccessTransformation of C andida guilliermondii wild‐type strains using the S taphylococcus aureus MRSA 252 ble gene as a phleomycin‐resistant marker
Author(s) -
Foureau Emilien,
Courdavault Vincent,
Simkin Andrew J.,
Sibirny Andriy A.,
Crèche Joël,
GiglioliGuivarc'h Nathalie,
Clastre Marc,
Papon Nicolas
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12034
Subject(s) - biology , staphylococcus aureus , gene , selectable marker , transformation (genetics) , yeast , coding region , microbiology and biotechnology , genetics , bacteria
We designed an efficient transformation system for C andida guilliermondii wild‐type strains. We demonstrated that the S taphylococcus aureus MRSA 252 ble coding sequence placed under the control of the yeast phosphoglycerate kinase gene transcription–regulating regions confers phleomycin resistance to transformed C . guilliermondii cells. To illustrate the potential of this drug‐resistant cassette, we carried out the disruption of the C . guilliermondii ADE2 gene. This new dominant selectable marker represents a powerful tool to study the function of various genes in this yeast of clinical and biotechnological interest.