Open AccessThe transcriptional control machinery as well as the cell wall integrity and its regulation are involved in the detoxification of the organic solvent dimethyl sulfoxide in S accharomyces cerevisiae
Author(s) -
Zhang Lilin,
Liu Ningning,
Ma Xiao,
Jiang Linghuo
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12022
Subject(s) - biology , saccharomyces cerevisiae , gene , dimethyl sulfoxide , transcriptional regulation , detoxification (alternative medicine) , gene expression , microbiology and biotechnology , regulation of gene expression , yeast , biochemistry , mediator , genetics , chemistry , medicine , alternative medicine , organic chemistry , pathology
In the present study, we have identified 339 dimethyl sulfoxide ( DMSO )‐sensitive and nine DMSO ‐tolerant gene mutations in S accharomyces cerevisiae through a functional genomics approach. Twelve of these identified DMSO ‐sensitive mutations are of genes involved in the general control of gene expression mediated by the SWR 1 complex and the RNA polymerase II mediator complex, whereas 71 of them are of genes involved in the protein trafficking and vacuolar sorting processes. In addition, twelve of these DMSO ‐sensitive mutations are of genes involved in the cell wall integrity ( CWI ) and its regulation. DMSO ‐tolerant mutations are of genes mainly involved in the metabolism and the gene expression control. Therefore, the transcriptional control machinery, the CWI and its regulation as well as the protein trafficking and sorting process play critical roles in the DMSO detoxification in yeast cells.