Open AccessRelationships among genera of the Saccharomycotina ( A scomycota ) from multigene phylogenetic analysis of type species
Author(s) -
Kurtzman Cletus P.,
Robnett Christie J.
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12006
Subject(s) - biology , clade , ascomycota , yarrowia , type species , hypocreales , phylogenetic tree , phylogenetics , evolutionary biology , zoology , botany , genus , genetics , yeast , gene
Relationships among ascomycetous yeast genera (subphylum S accharomycotina , phylum A scomycota ) have been uncertain. In the present study, type species of 70 currently recognized genera are compared from divergence in the nearly entire nuclear gene sequences for large subunit r RNA , small subunit (SSU) r RNA , translation elongation factor‐1α, and RNA polymerase II , subunits 1 ( RPB 1) and 2 ( RPB 2). The analysis substantiates earlier proposals that all known ascomycetous yeast genera now assigned to the S accharomycotina represent a single clade. Maximum likelihood analysis resolved the taxa into eight large multigenus clades and four‐one‐ and two‐genus clades. Maximum parsimony and neighbor‐joining analyses gave similar results. Genera of the family Saccharomycetaceae remain as one large clade as previously demonstrated, to which the genus C yniclomyces is now assigned. P ichia , S aturnispora , K regervanrija , D ekkera , O gataea and A mbrosiozyma are members of a single large clade, which is separate from the clade that includes B arnettozyma , C yberlindnera , P haffomyces , S tarmera and W ickerhamomyces . Other clades include K odamaea , M etschnikowia , D ebaryomyces , C ephaloascus and related genera, which are separate from the clade that includes Z ygoascus , T richomonascus , Y arrowia and others. This study once again demonstrates that there is limited congruence between a system of classification based on phenotype and a system determined from DNA sequences.