Open AccessDe novo generation of a phosphate starvation‐regulated promoter in C andida glabrata
Author(s) -
Kerwin Christine L.,
Wykoff Dennis D.
Publication year - 2012
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12000
Subject(s) - biology , promoter , transcription factor , gene , candida glabrata , transcription (linguistics) , coactivator , saccharomyces cerevisiae , microbiology and biotechnology , phosphatase , gene duplication , regulation of gene expression , genetics , phosphorylation , gene expression , candida albicans , linguistics , philosophy
What steps are required for a promoter to acquire regulation by an environmental condition? We address this question by examining a promoter in C andida glabrata that is regulated by phosphate starvation and the transcription factor P ho4. The gene PMU2 encodes a secreted acid phosphatase that resulted from gene duplication events not present in other A scomycetes , and only this gene of the three paralogs has acquired P ho4 regulation. We observe that the PMU2 promoter from C . glabrata is not functional in S accharomyces cerevisiae , which is surprising because it is regulated by P ho4, and P ho4 is regulated in a similar manner in both species – through phosphorylation and localization. Additionally, we determine that phosphate starvation‐regulated promoters in C . glabrata do not require the coactivator P ho2, which is essential to the phosphate starvation response in S . cerevisiae . We define a region of the PMU2 promoter that is important for P ho4 regulation, and this promoter region does not contain the canonical CACGTX sequence that S c P ho4 utilizes for phosphate starvation‐dependent transcription. However, C g P ho4 utilizes CACGTX in the CgPHO84 promoter, as mutation of this sequence decreases transcription. We conclude that the acquisition of PMU2 has expanded the binding specificity of C g P ho4 relative to S c P ho4.