Nonlinear Modeling on Protein Kinase C(PKC)-Epsilon Inverse Regulation of Stress Fibers in Oncogenically Transformed Cells

Phorbol 12-myristate 13-acetate (PMA), a tumor promoter, provides effective means of reversibly converting tissue culture cells to a state which resembles that of transformed cells. PKC isozymes can be activated by PMA and cause reorganization of actin-containing structures. The purpose of the current research is to analyze the pattern in which PKC mediates dissolution of stress fibers (SFs) by a mathematical model. Several mathematical models were tried, including linear and nonlinear approximations, in order to establish the relationship between PKCand SF accumulation in 1000W cells. Applying a non-linear approximation and using a bisection algorithm, an exponential function was derived to show how PKCinversely controlled SFs. We use RNA interference (RNAi) technology to knock down the PKCexpression in order to validate this mathematical model. In the time course of SF formation following PMA exposure, a special feature of parallel arrays showed in cells with PKCsiRNA transfection.