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White blood cells (WBC) play a significant role in human immune system, so WBC detection is a meaningful work. In this paper, we propose a novel framework which combines Fourier ptychographic microscopy (FPM) and SO-you only look once (YOLO) for WCB detection. FPM is a recently developed microscope technology which can achieve high-resolution, wide-field, and quantitative phase imaging at the same time without mechanical moving and phase measurement. With the FPM, we can get high resolution, wide field-of-view blood cell images at one time. After obtaining high-resolution images, we propose SO-YOLO as our detection method. As a convolutional neural network, the SO-YOLO outperforms the state-of-the-art detection methods both in accuracy and speed. The proposed framework in this paper can be used in clinical diagnose, because the experiment setup is flexible and the detection is fast. The data set in this paper is made with FPM by ourselves. Our data set contains 1000 color high resolution images and all the WBC has been detected and counted by a human expert. The detection results by the experts are regarded as the ground truth. The experiment results show that the SO-YOLO performs well in detecting small objects compared with other methods.

SO-YOLO Based WBC Detection With Fourier Ptychographic Microscopy