Open AccessPurification, crystallization and preliminary X‐ray analysis of IMP‐18, a class B carbapenemase from Pseudomonas aeruginosa
Author(s) -
Furuyama Takamitsu,
Ishii Yoshikazu,
Ohya Norimasa,
Tateda Kazuhiro,
Hanson Nancy D.,
ShimizuIbuka Akiko
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113030480
Subject(s) - pseudomonas aeruginosa , crystallization , tetragonal crystal system , crystallography , molecule , resolution (logic) , hydrolysis , escherichia coli , crystal (programming language) , chemistry , pseudomonas , stereochemistry , materials science , crystal structure , biology , bacteria , biochemistry , organic chemistry , genetics , gene , artificial intelligence , computer science , programming language
Class B β‐lactamases are known as metallo‐β‐lactamases (MBLs) and they hydrolyze most β‐lactams, including carbapenems. IMP‐18, an MBL cloned from Pseudomonas aeruginosa , was overexpressed, purified and crystallized by vapour diffusion for X‐ray crystallographic analysis. Preliminary X‐ray analysis showed that the crystal diffracted to 2.4 Å resolution and belonged to the tetragonal space group P 4 1 2 1 2, with unit‐cell parameters a = b = 120.77, c = 96.54 Å, α = β = γ = 90°, suggesting the presence of two molecules in the asymmetric unit.