Open AccessCrystallization and preliminary X‐ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4
Author(s) -
Wen Jinjin,
Li Zhenzhen,
He Guangzheng,
Xu Shujing,
Zhao Baohua,
Zhu Xianming,
Dong Hui,
Ju Jiansong
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113025426
Subject(s) - triclinic crystal system , crystallization , crystallography , resolution (logic) , alanine , tris , x ray crystallography , chemistry , x ray , materials science , crystal structure , nuclear chemistry , diffraction , amino acid , biochemistry , organic chemistry , optics , physics , artificial intelligence , computer science
Alanine dehydrogenase (OF4Ald) from the alkaliphilic Bacillus pseudofirmus OF4 was expressed and purified with a His 6 tag in a form suitable for X‐ray crystallographic analysis. Crystals were grown by the hanging‐drop vapour‐diffusion method at 289 K using a solution consisting of 0.1 M Tris–HCl pH 8.0, 0.2 M LiSO 4 , 22%( w / v ) PEG 3350. X‐ray diffraction data were collected to 2.8 Å resolution. The crystal belonged to the triclinic space group P 1, with unit‐cell parameters a = 88.04, b = 105.59, c = 120.53 Å, α = 88.37, β = 78.77, γ = 82.65°.