Open AccessCrystallization and preliminary X‐ray diffraction of the RNA demethylase ALKBH5
Author(s) -
Zhou Bin,
Han Zhifu
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113024858
Subject(s) - crystallization , crystallography , demethylase , x ray , diffraction , materials science , chemistry , biochemistry , physics , optics , dna , organic chemistry , histone
N6 ‐Methyladenosine (m6A) is a ubiquitous modification found in mammalian mRNA and long noncoding RNA. ALKBH5 is a member of the iron(II)‐ and 2‐oxoglutarate‐dependent AlkB oxygenase family and has been shown to catalyze the oxidative demethylation of N 6 ‐methyladenosine in RNA. The ALKBH5 protein was purified and crystallized using the hanging‐drop vapour‐diffusion method. The crystals diffracted to 2.4 Å resolution using synchrotron radiation. The crystals belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 57.456, b = 83.406, c = 92.909 Å, α = β = γ = 90.00° and one molecule in the asymmetric unit.