Overexpression, crystallization and preliminary X‐ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron

Bacteroides thetaiotaomicron BT0793, a putative xylose isomerase, was overexpressed in Escherichia coli , purified and crystallized using polyethylene glycol monomethyl ether 550 as the precipitant. X‐ray diffraction data were collected to 2.10 Å resolution at 100 K using synchrotron X‐rays. The crystal was found to belong to space group P 1, with unit‐cell parameters a = 96.3, b = 101.7, c = 108.3 Å, α = 82.8, β = 68.2, γ = 83.0°. The asymmetric unit contained eight subunits of xylose isomerase with a crystal volume per protein weight ( V M ) of 2.38 Å 3  Da −1 and a solvent content of 48.3%.