Open AccessCloning, expression, purification and preliminary X‐ray analysis of the dimerization domain of ethylene response sensor 1 (ERS1) from Arabidopsis thaliana
Author(s) -
Mayerhofer Hubert,
MuellerDieckmann Jochen
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113021751
Subject(s) - subfamily , ethylene , arabidopsis thaliana , resolution (logic) , cloning (programming) , signalling , domain (mathematical analysis) , diffraction , crystallography , x ray , chemistry , molecule , arabidopsis , biophysics , biology , stereochemistry , microbiology and biotechnology , biochemistry , physics , optics , organic chemistry , gene , computer science , mathematical analysis , mathematics , artificial intelligence , mutant , catalysis , programming language
Ethylene signalling is initiated by a group of membrane‐bound receptors with similarity to two‐component systems. ERS1 belongs, together with ETR1, to subfamily 1, which plays a predominant role in ethylene signalling. The dimerization domain of ERS1 was crystallized in space groups C 222 1 and P 2 1 2 1 2, with two and four molecules per asymmetric unit, respectively. The crystals diffracted X‐ray radiation to 1.9 Å resolution.