Open AccessCrystallization studies of the keratin‐like domain from Arabidopsis thaliana SEPALLATA 3
Author(s) -
Acajjaoui Samira,
Zubieta Chloe
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430911302006x
Subject(s) - mads box , egf like domain , arabidopsis thaliana , biology , arabidopsis , domain (mathematical analysis) , keratin , gene , transcription factor , microbiology and biotechnology , genetics , protein domain , mutant , mathematical analysis , mathematics
In higher plants, the MADS‐box genes encode a large family of transcription factors (TFs) involved in key developmental processes, most notably plant reproduction, flowering and floral organ development. SEPALLATA 3 (SEP3) is a member of the MADS TF family and plays a role in the development of the floral organs through the formation of multiprotein complexes with other MADS‐family TFs. SEP3 is divided into four domains: the M (MADS) domain, involved in DNA binding and dimerization, the I (intervening) domain, a short domain involved in dimerization, the K (keratin‐like) domain important for multimeric MADS complex formation and the C (C‐terminal) domain, a largely unstructured region putatively important for higher‐order complex formation. The entire K domain along with a portion of the I and C domains of SEP3 was crystallized using high‐throughput robotic screening followed by optimization. The crystals belonged to space group P 2 1 2 1 2, with unit‐cell parameters a = 123.44, b = 143.07, c = 49.83 Å, and a complete data set was collected to 2.53 Å resolution.