Open AccessCrystallization and preliminary X‐ray analysis of monalysin, a novel β‐pore‐forming toxin from the entomopathogen Pseudomonas entomophila
Author(s) -
Blemont Maryline,
Vincentelli Renaud,
Kellenberger Christine,
Opota Onya,
Lemaitre Bruno,
Roussel Alain,
Leone Philippe
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430911301885x
Subject(s) - monoclinic crystal system , crystallography , resolution (logic) , crystallization , monomer , derivative (finance) , x ray crystallography , diffraction , chemistry , materials science , crystal structure , physics , optics , polymer , organic chemistry , artificial intelligence , computer science , financial economics , economics
Monalysin was recently described as a novel pore‐forming toxin (PFT) secreted by the Drosophila pathogen Pseudomonas entomophila . Recombinant monalysin is multimeric in solution, whereas PFTs are supposed to be monomeric until target membrane association. Monalysin crystals were obtained by the hanging‐drop vapour‐diffusion method using PEG 8000 as precipitant. Preliminary X‐ray diffraction analysis revealed that monalysin crystals belonged to the monoclinic space group C 2, with unit‐cell parameters a = 162.4, b = 146.2, c = 144.4 Å, β = 122.8°, and diffracted to 2.85 Å resolution using synchrotron radiation. Patterson self‐rotation analysis and Matthews coefficient calculation indicate that the asymmetric unit contains nine copies of monalysin. Heavy‐atom derivative data were collected and a Ta 6 Br 14 cluster derivative data set confirmed the presence of ninefold noncrystallographic symmetry.