Open AccessExpression, purification, crystallization and preliminary X‐ray analysis of the extracellular sensory domain of DraK histidine kinase from Streptomyces coelicolor
Author(s) -
Yeo Kwon Joo,
Han YoungHyun,
Eo Yumi,
Cheong HaeKap
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113018769
Subject(s) - streptomyces coelicolor , extracellular , histidine kinase , crystallization , histidine , kinase , chemistry , streptomyces , biochemistry , crystallography , microbiology and biotechnology , biology , genetics , bacteria , enzyme , organic chemistry
The bacterium Streptomyces coelicolor produces useful antibiotics from its secondary metabolites. DraK is a sensory histidine kinase involved in the differential regulation of antibiotics in S. coelicolor through the DraR/DraK two‐component system. Here, the extracellular sensory domain of DraK was overexpressed in Escherichia coli , purified and crystallized using the sitting‐drop vapour‐diffusion method. The crystal diffracted to 2.2 Å resolution and belonged to space group C 222 1 , with unit‐cell parameters a = 41.91, b = 174.50, c = 145.25 Å, α = β = γ = 90°.