Open AccessCrystallization and preliminary X‐ray crystallographic analysis of MxaJ, a component of the methanol‐oxidizing system operon from the marine bacterium Methylophaga aminisulfidivorans MP T
Author(s) -
Choi Jin Myung,
Kang Jung Hun,
Lee DongWoo,
Kim Si Wouk,
Lee Sung Haeng
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113017983
Subject(s) - orthorhombic crystal system , methanol , operon , crystallography , crystallization , chemistry , electron transfer , oxidizing agent , crystal structure , crystal (programming language) , escherichia coli , biochemistry , organic chemistry , gene , computer science , programming language
The methanol‐oxidizing system (mox) is essential for methylotrophic bacteria to extract energy during the oxidoreduction reaction and consists of a series of electron‐transfer proteins encoded by the mox operon. One of the key enzymes is the α 2 β 2 methanol dehydrogenase complex (type I MDH), which converts methanol to formaldehyde during the 2e − transfer through the prosthetic group pyrroloquinoline quinone. MxaJ, a product of mxa J of the mox operon, is a component of the MDH complex and enhances the methanol‐converting activity of the MDH complex. However, the exact functional mechanism of MxaJ in the complex is not clearly known. To investigate the functional role of MxaJ in MDH activity, an attempt was made to determine its crystal structure. Diffraction data were collected from a selenomethionine‐substituted crystal to 1.92 Å resolution at the peak wavelength. The crystal belonged to the orthorhombic space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 37.127, b = 63.761, c = 99.246 Å. The asymmetric unit contained one MxaJ molecule with a calculated Matthews coefficient of 2.11 Å 3 Da −1 and a solvent content of 41.7%. Three‐dimensional structure determination of the MxaJ protein is currently in progress by the single‐wavelength anomalous dispersion technique and model building.