Open AccessExpression, purification, crystallization and preliminary crystallographic study of FtsA from methicillin‐resistant Staphylococcus aureus
Author(s) -
Fujita Junso,
Miyazaki Yuma,
Hirose Mika,
Nagao Chioko,
Mizohata Eiichi,
Matsumoto Yoshimi,
Mizuguchi Kenji,
Inoue Tsuyoshi,
Matsumura Hiroyoshi
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113017727
Subject(s) - crystallization , staphylococcus aureus , crystallography , microbiology and biotechnology , methicillin resistant staphylococcus aureus , chemistry , materials science , biology , bacteria , genetics , organic chemistry
FtsA from methicillin‐resistant Staphylococcus aureus (MRSA) was cloned, overexpressed and purified. The protein was crystallized using the sitting‐drop vapour‐diffusion technique. A cocrystal with β‐γ‐imidoadenosine 5′‐phosphate (AMPPNP; a nonhydrolysable ATP analogue) was grown using PEG 3350 as a precipitant at 293 K. X‐ray diffraction data were collected to a resolution of 2.3 Å at 100 K. The crystal belonged to the monoclinic space group P 2 1 , with unit‐cell parameters a = 75.31, b = 102.78, c = 105.90 Å, β = 96.54°. The calculated Matthews coefficient suggested that the asymmetric unit contained three or four monomers.