Open AccessPreliminary X‐ray crystallographic analysis of β‐carbonic anhydrase psCA3 from Pseudomonas aeruginosa
Author(s) -
Pinard Melissa,
Lotlikar Shalaka,
Patrauchan Marianna A.,
McKenna Robert
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113017594
Subject(s) - pseudomonas aeruginosa , crystallography , molecule , molecular replacement , chemistry , escherichia coli , resolution (logic) , crystal structure , stereochemistry , gene , biology , bacteria , biochemistry , genetics , organic chemistry , artificial intelligence , computer science
Pseudomonas aeruginosa is a Gram‐negative bacterium that causes life‐threatening infections in susceptible individuals and is resistant to most clinically available antimicrobials. Genomic and proteomic studies have identified three genes, pa0102 , pa2053 and pa4676 , in P. aeruginosa PAO1 encoding three functional β‐carbonic anhydrases (β‐CAs): psCA1, psCA2 and psCA3, respectively. These β‐CAs could serve as novel antimicrobial drug targets for this pathogen. X‐ray crystallographic structural studies have been initiated to characterize the structure and function of these proteins. This communication describes the production of two crystal forms ( A and B ) of β‐CA psCA3. Form A diffracted to a resolution of 2.9 Å; it belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 81.9, b = 84.9, c = 124.2 Å, and had a calculated Matthews coefficient of 2.23 Å 3 Da −1 assuming four molecules in the crystallographic asymmetric unit. Form B diffracted to a resolution of 3.0 Å; it belonged to space group P 2 1 2 1 2, with unit‐cell parameters a = 69.9, b = 77.7, c = 88.5 Å, and had a calculated Matthews coefficient of 2.48 Å 3 Da −1 assuming two molecules in the crystallographic asymmetric unit. Preliminary molecular‐replacement solutions have been determined with the PHENIX AutoMR wizard and refinement of both crystal forms is currently in progress.