Open AccessCrystallization and preliminary X‐ray diffraction studies of cyanuric acid hydrolase from Azorhizobium caulinodans
Author(s) -
Cho Seunghee,
Shi Ke,
Wackett Lawrence P.,
Aihara Hideki
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113017077
Subject(s) - cyanuric acid , crystallization , chemistry , crystallography , hydrolase , microbiology and biotechnology , materials science , biochemistry , enzyme , biology , organic chemistry , melamine
Cyanuric acid is synthesized industrially and forms during the microbial metabolism of s ‐triazine herbicides. Cyanuric acid is metabolized by some microorganisms via cyanuric acid hydrolase (CAH), which opens the s ‐triazine ring as a prelude to further metabolism. CAH is a member of the rare cyanuric acid hydrolase/barbiturase family. Here, the crystallization and preliminary X‐ray diffraction analysis of CAH from Azorhizobium caulinodans are reported. CAH was cocrystallized with barbituric acid, a close analog of cyanuric acid that is a tight‐binding competitive inhibitor. Crystals suitable for X‐ray diffraction experiments were grown in conditions containing PEG 8K or magnesium sulfate as precipitants. An X‐ray diffraction data set was collected from CAH–barbituric acid crystals to 2.7 Å resolution. The crystals were found to belong to space group I 4 1 22, with unit‐cell parameters a = b = 237.9, c = 105.3 Å, α = β = γ = 90°.