Open AccessMimer : an automated spreadsheet‐based crystallization screening system
Author(s) -
Brodersen Ditlev Egeskov,
Andersen Gregers Rom,
Andersen Christian Brix Folsted
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113014425
Subject(s) - crystallization , computer science , pipette , grid , process engineering , computer hardware , chemical engineering , chemistry , engineering , mathematics , geometry
In this paper, a simple low‐cost alternative to large commercial systems for preparing macromolecular crystallization conditions is described. Using an intuitive spreadsheet‐based approach, the system allows the rapid calculation of relevant pipetting volumes given known stock‐solution concentrations and incorporates the automatic design of custom crystallization screens via the incomplete‐factorial and grid‐screen approaches. Automated dispensing of the resulting crystallization screens is achieved using a generic and relatively inexpensive liquid handler.