Open AccessStructure of diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‐amino‐6‐(5‐phosphoribosylamino)uracil reductase from Acinetobacter baumannii
Author(s) -
Dawson Alice,
Trumper Paul,
Chrysostomou Georgios,
Hunter William N.
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430911301292x
Subject(s) - uracil , tetragonal crystal system , orthorhombic crystal system , acinetobacter baumannii , guanosine , reductase , chemistry , crystal structure , biochemistry , stereochemistry , enzyme , biology , crystallography , bacteria , genetics , dna , pseudomonas aeruginosa
The bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‐amino‐6‐(5‐phosphoribosylamino)uracil reductase (RibD) represents a potential antibacterial drug target. The structure of recombinant Acinetobacter baumannii RibD is reported in orthorhombic and tetragonal crystal forms at 2.2 and 2.0 Å resolution, respectively. Comparisons with orthologous structures in the Protein Data Bank indicated close similarities. The tetragonal crystal form was obtained in the presence of guanosine monophosphate, which surprisingly was observed to occupy the adenine‐binding site of the reductase domain.