Open AccessCrystallization and X‐ray diffraction analysis of an antifungal laticifer protein
Author(s) -
BrunoMoreno Frederico,
Sombra Basílio de Oliveira Raquel,
de Azevedo Moreira Renato,
Pinto Lobo Marina Duarte,
Teixeira de Freitas Cléverson Diniz,
Viana Ramos Márcio,
Barbosa Grangeiro Thalles,
Oliveira MonteiroMoreira Ana Cristina
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113011378
Subject(s) - ammonium sulfate , crystallization , chemistry , hepes , tetragonal crystal system , antifungal , nuclear chemistry , x ray crystallography , crystallography , crystal structure , chromatography , diffraction , biology , biochemistry , organic chemistry , microbiology and biotechnology , physics , optics
An osmotin (CpOsm) from the latex of Calotropis procera has been crystallized in both tetragonal and trigonal forms suitable for structure determination. Crystallographic studies of CpOsm are of great interest because limited information is available concerning the structure of latex proteins and CpOsm has previously been shown to interact with the spore membranes of some plant pathogenic fungi, thus impairing spore germination and hyphal growth. CpOsm crystals were grown using 0.1 M HEPES buffer pH 7.5, 26% PEG 4000, 0.2 M ammonium sulfate (space group P 4 3 ) or using 0.1 M HEPES buffer pH 7.5, 35% MPD, 0.7 M ammonium sulfate (space group P 3 1 12). X‐ray diffraction data were collected to 2.17 Å ( P 4 3 ) and 1.80 Å ( P 3 1 12) resolution and molecular‐replacement analyses produced initial phases for both crystal forms.