Open AccessCrystallization and preliminary structural analysis of dibenzothiophene monooxygenase (DszC) from Rhodococcus erythropolis
Author(s) -
Duan Xiaolu,
Zhang Liang,
Zhou Daming,
Ji Kaihua,
Ma Ting,
Shui Wenqing,
Li Guoqiang,
Li Xin
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113011172
Subject(s) - dibenzothiophene , rhodococcus , monooxygenase , flue gas desulfurization , chemistry , sulfur , crystal structure , hydrodesulfurization , stereochemistry , organic chemistry , enzyme , cytochrome p450
Dibenzothiophene (DBT) and its derivatives are typical sulfur compounds found in fossil fuels. These compounds show resistance to the hydrodesulfurization treatment that is commonly used in industry. Dibenzothiophene monooxygenase (DszC) is responsible for the oxidation of DBT, which is the first and the rate‐limiting step in the DBT enzymatic desulfurization 4S pathway. In this study, the crystal structure of DszC from Rhodococcus erythropolis DS‐3 is reported. The crystal of native DszC belonged to space group P 1, with unit‐cell parameters a = 96.16, b = 96.27, c = 98.56 Å, α = 81.03, β = 67.57, γ = 85.84°. To determine the phase, SAD X‐ray diffraction data were collected from a SeMet‐derivative DszC crystal, which also belonged to space group P 1, with unit‐cell parameters a = 95.379, b = 95.167, c = 94.891 Å, α = 87.046, β = 70.536, γ = 79.738°. Further structural analysis of DszC is in progress.