Cloning, expression, purification, crystallization and X‐ray crystallographic analysis of Rv2606c from Mycobacterium tuberculosis H37Rv

Tuberculosis is a widespread and deadly infectious disease, and one third of the human population is already infected. Vitamin B 6 is known to be synthesized through consecutive reactions mediated by pyridoxal biosynthesis lyase (PdxS) and glutamine amidotransferase (PdxT). The gene product Rv2606c, the PdxS pyridoxal biosynthesis lyase from Mycobacterium tuberculosis , was crystallized using the hanging‐drop vapour‐diffusion method in the presence of 8%( w / v ) PEG 8000, 0.1  M 3‐(cyclohexylamino)‐1‐propanesulfonic acid pH 10.5 and 0.2  M sodium chloride at 295 K. X‐ray diffraction data were collected to a maximum resolution of 1.7 Å on a synchrotron beamline. The crystal belonged to space group I 222 or I 2 1 2 1 2 1 , with unit‐cell parameters a = 110.75, b = 126.08, c  = 180.82 Å, α = β = γ = 90°. With three molecules per asymmetric unit, the crystal volume per unit protein weight ( V M ) was 3.79 Å 3  Da −1 .