Open AccessCrystallization and initial X‐ray data of abscisic acid receptor PYL3 in the presence of (−)‐ABA
Author(s) -
Zhang Xingliang,
Zhang Qi,
Wang Guoqiang
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113008051
Subject(s) - abscisic acid , stereospecificity , crystallization , ligand (biochemistry) , receptor , chemistry , resolution (logic) , microbiology and biotechnology , stereochemistry , biochemistry , biophysics , biology , organic chemistry , gene , catalysis , computer science , artificial intelligence
Abscisic acid (ABA) modulates many complicated developmental processes and responses to environmental stimuli. Recently, several (+)‐ABA signalling mechanisms by the RCAR/PYR1/PYL family of proteins (PYLs) have been proposed. However, the mechanism of the recognition and binding of the unnatural ligand (−)‐ABA by PYLs has not yet been elucidated. In the present study, the expression, purification and crystallization of PYL3 in complex with (−)‐ABA are reported. Diffraction data were refined to 2.65 Å resolution for this complex in space group P 6 5 . These findings will help to explain the stereospecificity of PYLs for (−)‐ABA and to explore the selective ABA agonists.