Open AccessStructure of the hypothetical DUF1811‐family protein GK0453 from Geobacillus kaustophilus HTA426
Author(s) -
Padmanabhan Balasundaram,
Nakamura Yoshihiro,
Antonyuk Svetlana V.,
Strange Richard W.,
Hasnain S. Samar,
Yokoyama Shigeyuki,
Bessho Yoshitaka
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113003369
Subject(s) - nucleic acid , molecular replacement , crystal structure , chemistry , crystallography , homology (biology) , amino acid residue , biochemistry , stereochemistry , biology , amino acid , peptide sequence , gene
The crystal structure of a conserved hypothetical protein, GK0453, from Geobacillus kaustophilus has been determined to 2.2 Å resolution. The crystal belonged to space group P 4 3 2 1 2, with unit‐cell parameters a = b = 75.69, c = 64.18 Å. The structure was determined by the molecular‐replacement method and was refined to a final R factor of 22.6% ( R free = 26.3%). Based on structural homology, the GK0453 protein possesses two independent binding sites and hence it may simultaneously interact with two proteins or with a protein and a nucleic acid.