Open AccessCrystallization and preliminary X‐ray diffraction analysis of the Nif3‐family protein MJ0927 from Methanocaldococcus jannaschii
Author(s) -
Kuan ShuMin,
Chen HsiaoChi,
Huang ChiHung,
Chang ChiHuang,
Chen ShengChia,
Yang Chia Shin,
Chen Yeh
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112049408
Subject(s) - orthorhombic crystal system , crystallography , crystallization , resolution (logic) , diffraction , biology , crystal structure , materials science , chemistry , physics , optics , computer science , organic chemistry , artificial intelligence
MJ0927 is a member of the Nif3 family and is widely distributed across living organisms. Although several crystal structures of Nif3 proteins have been reported, structural information on archaeal Nif3 is still limited. To understand the structural differences between bacterial and archaeal Nif3 proteins, MJ0927 from Methanocaldococcus jannaschii was purified and crystallized using the sitting‐drop vapour‐diffusion method. The crystals diffracted to a resolution of 2.47 Å and belonged to the orthorhombic space group C 222, with unit‐cell parameters a = 81.21, b = 172.94, c = 147.42 Å. Determination of this structure may provide insights into the function of MJ0927.