Open AccessOverexpression, crystallization and preliminary X‐ray crystallographic analysis of the phosphotriesterase from Mycobacterium tuberculosis
Author(s) -
Zhang Liang,
Chen Ruyi,
Dong Zhe,
Li Xin
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112049032
Subject(s) - crystallization , mycobacterium tuberculosis , dimer , crystal structure , molecule , crystallography , crystal (programming language) , chemistry , tuberculosis , materials science , organic chemistry , medicine , computer science , pathology , programming language
Organophosphates (OPs) are extremely toxic compounds that are used as insecticides or even as chemical warfare agents. Phosphotriesterases (PHPs) are responsible for the detoxification of OPs by catalysing their degradation. Almost 100 PHP structures have been solved to date, yet the crystal structure of the phosphotriesterase from Mycobacterium tuberculosis (mPHP) remains unavailable. This study reports the first crystallization of mPHP. The crystal belonged to space group C 222 1 , with unit‐cell parameters a = 68.03, b = 149.60, c = 74.23 Å, α = β = γ = 90°. An analytical ultracentrifugation experiment suggested that mPHP exists as a dimer in solution, even though one molecule is calculated to be present in the asymmetric unit according to the structural data.