Open AccessExpression, purification, crystallization and preliminary X‐ray analysis of NAD(P)H‐dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver
Author(s) -
Yoneda Kazunari,
Fukuda Yudai,
Shibata Takeshi,
Araki Tomohiro,
Nikki Takahiro,
Sakuraba Haruhiko,
Ohshima Toshihisa
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112046453
Subject(s) - nad+ kinase , monoclinic crystal system , reductase , resolution (logic) , crystallization , chemistry , polyethylene glycol , crystallography , enzyme , biochemistry , crystal structure , organic chemistry , artificial intelligence , computer science
An NAD(P)H‐dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver was crystallized using the sitting‐drop vapour‐diffusion method with polyethylene glycol 300 as the precipitant. The crystals belonged to the monoclinic space group C 2, with unit‐cell parameters a = 104.26, b = 81.32, c = 77.27 Å, β = 119.43°, and diffracted to 1.86 Å resolution on beamline NE3A at the Photon Factory. The overall R merge was 5.4% and the data completeness was 99.4%.