Open AccessCrystallization and preliminary X‐ray crystallographic analysis of UDP‐glucuronic acid:flavonol‐3‐ O ‐glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera
Author(s) -
Mizohata Eiichi,
Okuda Takuma,
Hatanaka Seika,
Nakayama Taisuke,
Horikawa Manabu,
Nakayama Toru,
Ono Eiichiro,
Inoue Tsuyoshi
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112045095
Subject(s) - glucuronic acid , chemistry , crystallography , crystallization , vitis vinifera , x ray , stereochemistry , biochemistry , botany , organic chemistry , biology , optics , polysaccharide , physics
Grapevine ( Vitis vinifera ) glycosyltransferase 5 (VvGT5) is a UDP‐glucuronic acid:flavonol‐3‐ O ‐glucuronosyltransferase that catalyses the 3‐ O ‐specific glucuronosylation of flavonols using UDP‐glucuronic acid as a sugar donor to produce flavonol 3‐ O ‐glucosides, which are important bioactive phytochemicals. Recombinant VvGT5 expressed in Escherichia coli cells was purified and crystallized by the sitting‐drop vapour‐diffusion method. A full set of X‐ray diffraction data was collected to 2.2 Å Bragg spacing from a single crystal using a synchrotron‐radiation source. The crystal was hexagonal, belonging to space group P 6 1 22, with unit‐cell parameters a = b = 102.70, c = 535.92 Å. The initial phases were determined by the molecular‐replacement method.