Open AccessCrystallization, optimization and preliminary X‐ray characterization of a metal‐dependent PI‐PLC from Streptomyces antibioticus
Author(s) -
Jackson Michael R.,
Selby Thomas L.
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112041371
Subject(s) - crystallization , characterization (materials science) , metal , pi , materials science , nuclear chemistry , chemistry , metallurgy , nanotechnology , organic chemistry , biochemistry
A recombinant metal‐dependent phosphatidylinositol‐specific phospholipase C (PI‐PLC) from Streptomyces antibioticus has been crystallized by the hanging‐drop method with and without heavy metals. The native crystals belonged to the orthorhombic space group P 222, with unit‐cell parameters a = 41.26, b = 51.86, c = 154.78 Å. The X‐ray diffraction results showed significant differences in the crystal quality of samples soaked with heavy atoms. Additionally, drop pinning, which increases the surface area of the drops, was also used to improve crystal growth and quality. The combination of heavy‐metal soaks and drop pinning was found to be critical for producing high‐quality crystals that diffracted to 1.23 Å resolution.