Open AccessCrystallization and diffraction of an Isl1–Ldb1 complex
Author(s) -
Gadd Morgan S.,
Jacques David A.,
Guss J. Mitchell,
Matthews Jacqueline M.
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112040031
Subject(s) - orthorhombic crystal system , crystallization , crystallography , resolution (logic) , diffraction , chemistry , materials science , physics , computer science , optics , crystal structure , organic chemistry , artificial intelligence
A stable intramolecular complex comprising the LIM domains of the LIM‐homeodomain protein Isl1 tethered to a peptide region of Ldb1 has been engineered, purified and crystallized. The orthorhombic crystals belonged to space group P 222 1 , with unit‐cell parameters a = 57.2, b = 56.7, c = 179.8 Å, and diffracted to 3.10 Å resolution.